Biofunctionalized nanoneedles for the direct and site-selective delivery of probes into living cells
نویسندگان
چکیده
منابع مشابه
nano-rods zno as an efficient catalyst for the synthesis of chromene phosphonates, direct amidation and formylation of amines
چکیده ندارد.
Selective labeling of proteins with chemical probes in living cells.
Selective labeling of proteins with small molecules introduces novel chemical and physical properties into proteins, enabling the target protein to be investigated or manipulated with various techniques. Different methods for labeling proteins in living cells have been developed by using protein domains, small peptides, or single amino acids. Their application in cells and in vivo has yielded n...
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The current arsenal of tools and methods for the continuous monitoring and imaging of redox metabolic pathways in the context of intact cells is limited. Fluorogenic substrates allow for direct measurement of enzyme activity in situ; however, in contrast to proteases and exo-glycosidases, there are no simple guidelines for the design of selective probes for redox metabolic enzymes. Here, we int...
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ABSTRACT: OBJECTIVE: To express recombinant human CYPs (2C8*1,*2,*3,*4, 2C9*1,*3,*13,*16, and 2D6*1,*10), determine their expression levels and activity. Then study their enzyme kinetic characteristics on stereoselective N-demethylation of fluoxetine (FLX). METHODS:. Recombinant human CYPs were prepared with the Spodopterafrugiperda Sf9 cell system. The recombinant human CYPs were subjected to ...
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ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - General Subjects
سال: 2011
ISSN: 0304-4165
DOI: 10.1016/j.bbagen.2010.05.005